Bonilla E
Clin Chem. 1978 Mar;24(3):471-4.
I describe a method for determining manganese in biological tissues by flameless atomic absorption spectrophotometry, It is simple to perform and does not require prior extraction with solvents. Small tissue samples are dried for 48 h at 110 degrees C and dissolved in concentrated nitric acid; the clear acid solution is introduced into the furnace and the sequential drying (120 degrees C, 20 s), charring (800 degrees C, 70 s), and atomization (2400 degrees C, 8 s) are automatically performed. The technique of standard additions was used ot overcome matrix effect. The detection limit is 0.86 X 10(-11) g of Mn. The CV is 4.7%. Only sodium and potassium interfere at 500-fold concentrations, and their interferences were overcome by using the Deuterium Arc Background Corrector.
我描述了一种通过无火焰原子吸收分光光度法测定生物组织中锰的方法。该方法操作简单，无需事先用溶剂萃取。将小组织样本在110℃下干燥48小时，然后溶于浓硝酸；将清澈的酸溶液引入炉中，自动进行依次干燥（120℃，20秒）、炭化（800℃，70秒）和原子化（2400℃，8秒）。采用标准加入法克服基体效应。检测限为0.86×10⁻¹¹克锰。变异系数为4.7%。只有钠和钾在浓度为500倍时产生干扰，使用氘弧背景校正器可克服它们的干扰。
