Department of Nutrition, University of California, 95616, Davis, CA.
Biol Trace Elem Res. 1981 Jun;3(2):107-15. doi: 10.1007/BF02990451.
A multitude of methods exists at present for the solubilization of biological tissues for atomic absorption analysis. We have examined several common methods of wet ashing using NBS bovine liver in order to determine which acids, acid combinations, or bases should be used as digesting agents for accurate and precise measurement of iron, copper, zinc, and manganese. Nitric acid proved to be the most effective wet ashing agent. With nitric acid, mean concentrations for iron, copper, and zinc differed from NBS certified values by less than 1.5% while those for manganese differed by 4%.
目前存在许多用于生物组织溶解以进行原子吸收分析的方法。我们已经研究了几种常用的湿式灰化方法,使用 NBS 牛肝来确定应该使用哪些酸、酸组合或碱作为消化剂,以实现铁、铜、锌和锰的准确和精密测量。硝酸被证明是最有效的湿式灰化剂。使用硝酸时,铁、铜和锌的平均浓度与 NBS 认证值的差异小于 1.5%,而锰的差异为 4%。