Bolin S R, Turek J J, Runnels L J, Gustafson D P
Am J Vet Res. 1983 Jun;44(6):1036-9.
Porcine embryos (n = 93) were incubated on cell monolayers that had been previously inoculated with pseudorabies virus, porcine parvovirus (PPV), or each of 2 porcine enteroviruses. After 2, 24, or 48 hours of incubation, the embryos were fixed in glutaraldehyde and examined by electron microscopic procedures. It was found that pseudorabies virus adsorbed to the zona pellucida (ZP) and entered sperm tracks in the ZP. The PPV and both enteroviruses entered pores in the ZP and were associated with sperm that were at or near the outer surface of the ZP. In addition, PPV was seen enmeshed in cellular debris on the outer surface of the ZP. Evidence of a productive viral infection of the blastomeres of the embryos was not found.
将93个猪胚胎置于预先接种了伪狂犬病病毒、猪细小病毒（PPV）或两种猪肠道病毒之一的细胞单层上进行培养。培养2、24或48小时后，将胚胎用戊二醛固定，并通过电子显微镜程序进行检查。结果发现，伪狂犬病病毒吸附在透明带（ZP）上，并进入ZP中的精子通道。PPV和两种肠道病毒都进入了ZP中的孔隙，并与位于ZP外表面或靠近外表面的精子相关联。此外，还观察到PPV缠绕在ZP外表面的细胞碎片中。未发现胚胎卵裂球有病毒感染增殖的证据。
