Mercer D W
Clin Chem. 1978 Mar;24(3):480-2.
Lactate dehydrogenase (LD) isoenzymes 1 and 2 in human serum were separated on a column of diethylaminoethyl-Sephadex. Samples layered on mini-columns were eluted with buffered sodium chloride (100, 150, and 200 mmol/liter). Lactate dehydrogenase activity in column effluents was measured by the Wacker method, and their isoenzyme content was evaluated by electrophoresis on polyacrylamide gel. Results for column-fractionated LD-1 and LD-2 were expressed in two ways: LD-1/LD-2 ratios and total LD-1 + LD-2 activities. The former is a more specific indicator of myocardial infarction than the latter. Sera from 10 patients with acute myocardial infarction (increased creatine kinease isoenzyme MB activity) exhibited ratios in the range of 0.92 to 1.56, ratios for 10 patients without heart disease (normal creatine kinase MB) ranged from 0.33 to 0.69.
人血清中的乳酸脱氢酶（LD）同工酶1和2在二乙氨基乙基-葡聚糖凝胶柱上进行分离。加样于微型柱上的样品用缓冲氯化钠（100、150和200 mmol/升）洗脱。柱流出液中的乳酸脱氢酶活性用瓦克法测定，其同工酶含量通过聚丙烯酰胺凝胶电泳进行评估。柱分级分离的LD-1和LD-2的结果以两种方式表示：LD-1/LD-2比值和总LD-1 + LD-2活性。前者是比后者更特异的心肌梗死指标。10例急性心肌梗死患者（肌酸激酶同工酶MB活性升高）的血清比值在0.92至1.56范围内，10例无心脏病患者（肌酸激酶MB正常）的比值在0.33至0.69范围内。
