Dayan D, Binderman I, Mechanic G L
Arch Oral Biol. 1983;28(2):185-7. doi: 10.1016/0003-9969(83)90126-7.
Collagenolytic activity in human carious and non-carious dentine matrix was compared. Results confirmed the presence of latent collagenase in demineralized dentine and indicated a slow rate of degradation of collagen substrate. Collagenolytic activity was enhanced with the addition of trypsin-TPCK to the demineralized dentine. More activity was observed in the carious dentine, suggesting the presence of collagenase activators or partial enzymic destruction of the inhibitor in the collagen-collagenase-inhibitor complex. It seems that during dentine development collagenase-inhibitor complex is secreted and bound to collagen-dentine matrix, and the enzyme can be activated during the process of dental caries.
比较了人类龋坏和非龋坏牙本质基质中的胶原olytic活性。结果证实脱矿牙本质中存在潜伏性胶原酶，并表明胶原底物的降解速度较慢。向脱矿牙本质中添加胰蛋白酶-TPCK可增强胶原olytic活性。在龋坏牙本质中观察到更多活性，提示胶原-胶原酶-抑制剂复合物中存在胶原酶激活剂或抑制剂的部分酶解破坏。在牙本质发育过程中，胶原酶-抑制剂复合物似乎被分泌并结合到胶原-牙本质基质上，并且该酶在龋齿过程中可被激活。
