Ashwini Ajay, Dineshkumar Thayalan, Rameshkumar Annasamy, Swarnalakshmi Raman, Shahnaz Ahmed, Nagarathinam A E, Rajkumar Krishnan
Post Graduate Student, Department of Oral Pathology and Microbiology, SRM Dental College, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
Associate Professor, Department of Oral Pathology and Microbiology, SRM Dental College, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
J Clin Exp Dent. 2020 Feb 1;12(2):e108-e115. doi: 10.4317/jced.56144. eCollection 2020 Feb.
Dentin caries involves dissolution of minerals which eventually leads to degradation of organic matrix. This degradation which was thought to be by bacterial proteases is now considered to be orchestrated by endogenous collagenases such as Matrix Metalloproteinases (MMPs). This paper aims to estimate the salivary levels of MMP-8 in dental caries and also to asses the various risk factors that contribute to the formation of dental caries.
A random sample of 75 adults aged 18-35 were included and categorized based on the number of caries lesions (MCL). Standard clinical examinations were performed, and stimulated saliva was collected and analyzed for concentrations of MMP-8 using enzyme-linked immunosorbent assay. Caries risk factors were assessed using a chair-side kit. Correlation of MMP-8 in varying MCL using Spearman's correlation was done. Multiple linear regression analysis was done to asses the relationship between various caries risk factors with MMP-8 and MCL as dependent variable.
The study results showed a statistically significant higher concentration of MMP-8 in carious group (MCL 1-2) and MCL ≥3 compared to non-carious group. On correlating, the levels of MMP-8 were seen to be higher in MCL ≥3 than in MCL = 0 and MCL 1-2. The mean MMP-8 of controls, MCL 1-2, and MCL ≥3 were 131.34ng/ml, 230.14ng/ml, and 391.91ng/ml respectively. Multiple linear regression analysis with MMP-8 as the dependent variable revealed caries, buffer capacity and count as significant variables. Using MCL as the dependent variable the only significant variable was MMP-8 levels.
The study suggests that subjects with caries have elevated MMP-8 levels compared with subjects with no carious lesions. There is also a positive correlation between the number of carious lesions and MMP-8 levels suggesting that MMP-8 plays an important role in the degradation of dentin and causes progression of caries. Caries, Dentin, Enzyme-linked Immunosorbent Assay, MMP-8.
牙本质龋涉及矿物质溶解，最终导致有机基质降解。这种曾被认为是由细菌蛋白酶引起的降解，现在被认为是由内源性胶原酶如基质金属蛋白酶（MMPs）精心调控的。本文旨在评估龋齿患者唾液中MMP - 8的水平，并评估导致龋齿形成的各种风险因素。
纳入75名年龄在18 - 35岁的成年人随机样本，根据龋损数量（MCL）进行分类。进行标准临床检查，收集刺激唾液，并用酶联免疫吸附测定法分析MMP - 8的浓度。使用椅旁试剂盒评估龋齿风险因素。采用Spearman相关性分析不同MCL中MMP - 8的相关性。以MMP - 8和MCL为因变量，进行多元线性回归分析以评估各种龋齿风险因素之间的关系。
研究结果显示，与无龋组相比，龋病组（MCL 1 - 2）和MCL≥3组中MMP - 8的浓度在统计学上显著更高。相关性分析显示，MCL≥3组中MMP - 8的水平高于MCL = 0组和MCL 1 - 2组。对照组、MCL 1 - 2组和MCL≥3组的MMP - 8平均水平分别为131.34ng/ml、230.14ng/ml和391.91ng/ml。以MMP - 8为因变量的多元线性回归分析显示，龋齿、缓冲能力和计数为显著变量。以MCL为因变量，唯一显著的变量是MMP - 8水平。
该研究表明，与无龋损的受试者相比，有龋损的受试者MMP - 8水平升高。龋损数量与MMP - 8水平之间也存在正相关，表明MMP - 8在牙本质降解中起重要作用，并导致龋齿进展。龋齿、牙本质酶联免疫吸附测定、MMP - 8 。
