Huang H F, Marshall G R
Biol Reprod. 1983 Jun;28(5):1163-72. doi: 10.1095/biolreprod28.5.1163.
Vitamin A deficiency (VAD) was induced in male Sprague-Dawley rats by feeding them a vitamin A-deficient diet after weaning and subsequently supplementing their diet with retinoic acid. The serum vitamin A levels of these rats were at a minimum by 60 days of age. Although spermatogenesis can proceed normally during the early phase of vitamin A deficiency, a small number of late spermatids were not released from the seminiferous epithelium at the end of Stage VIII of the cycle of the seminiferous epithelium but rather at a later stage. This observation suggests a delay in spermiation. This delayed spermiation was noted as early as 50 days of age and persisted in rats even after 60 days of vitamin A replacement. These results demonstrate that in rats the timing of spermiation can be retarded by vitamin A deficiency. Although the mechanisms causing this delay in spermiation are not known, the vitamin A-deficient rat provides a useful model system for studies on the regulation of spermiation.
通过在雄性斯普拉格-道利大鼠断奶后喂食维生素A缺乏饮食，随后在其饮食中补充视黄酸，诱导其维生素A缺乏（VAD）。这些大鼠的血清维生素A水平在60日龄时降至最低。虽然在维生素A缺乏的早期阶段精子发生可以正常进行，但少数晚期精子细胞在生精上皮周期的第八阶段结束时并未从生精上皮释放，而是在更晚的阶段释放。这一观察结果表明精子释放延迟。这种延迟的精子释放早在50日龄时就已被注意到，并且在维生素A替代60天后大鼠中仍然存在。这些结果表明，在大鼠中，精子释放的时间可以被维生素A缺乏所延迟。虽然导致这种精子释放延迟的机制尚不清楚，但维生素A缺乏的大鼠为研究精子释放的调节提供了一个有用的模型系统。
