McMorris F A
J Neurochem. 1983 Aug;41(2):506-15. doi: 10.1111/j.1471-4159.1983.tb04768.x.
Cyclic AMP (cAMP) is known to induce the activity of the myelin enzyme 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP; EC 3.1.4.37) in C6 rat glioma cells. This report shows that CNP is also inducible in oligodendrocytes explanted from 1-day-old rat cerebrum and grown in tissue culture. Induction was observed after a 1-day treatment with 1 mM N6, O2-dibutyryl cyclic AMP (dbcAMP) and was maximal after 5 days, reaching 200-240% of control. Induction was observed both in mixed cerebral cell cultures containing oligodendrocytes and astrocytes, and in purified cultures of oligodendrocytes prepared by a differential shakeoff procedure. Addition of dbcAMP to the cultures 3-9 days after the cells were explanted from rat brain induced CNP activity, but no induction was observed when dbcAMP treatment was begun 13 or more days after explanation. These results demonstrate that one component of myelin, CNP, is inducible in oligodendrocytes by a cAMP-mediated mechanism, and suggest a role for cAMP in the regulation of the myelin-associated functions of oligodendrocytes.
已知环磷酸腺苷（cAMP）可诱导C6大鼠胶质瘤细胞中髓磷脂酶2'，3'-环核苷酸3'-磷酸水解酶（CNP；EC 3.1.4.37）的活性。本报告表明，从1日龄大鼠大脑中分离并在组织培养中生长的少突胶质细胞中，CNP也可被诱导。在用1 mM N6，O2-二丁酰环磷酸腺苷（dbcAMP）处理1天后观察到诱导作用，5天后达到最大值，达到对照的200-240%。在含有少突胶质细胞和星形胶质细胞的混合脑细胞培养物以及通过差异摇落法制备的少突胶质细胞纯化培养物中均观察到诱导作用。在细胞从大鼠脑中移出3-9天后向培养物中添加dbcAMP可诱导CNP活性，但在移出13天或更长时间后开始dbcAMP处理时未观察到诱导作用。这些结果表明，髓磷脂的一个成分CNP可通过cAMP介导的机制在少突胶质细胞中被诱导，并提示cAMP在少突胶质细胞髓磷脂相关功能的调节中起作用。
