Immunofluorescence demonstration of 2':3'-cyclic-nucleotide 3'-phosphodiesterase in cultured oligodendrocytes of mouse, rat, calf and human.

Previous biochemical studies have shown that high enzyme activity of 2':3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) is found in isolated myelin and oligodendrocytes. We report here the specific and intense immunofluorescence staining of cultured oligodendrocytes obtained from the brains of mouse, rat, calf and human by rabbit antiserum specific for purified bovine CNP. Astrocytes and fibroblasts present in the cultures were negative for the immunostaining. The specificity of the CNP immunoreactivity was confirmed by blocking the reaction by prior absorption of the antiserum by purified CNP.