Richardson P J
J Neurochem. 1983 Sep;41(3):640-8. doi: 10.1111/j.1471-4159.1983.tb04789.x.
Nerve terminals prepared from rat cortex and hippocampus were loaded with seven radioactive putative neurotransmitters (serotonin, noradrenaline, dopamine, gamma-aminobutyric acid, aspartate, glutamate, and taurine). The release of these transmitters, choline acetyltransferase, 3,4-dihydroxyphenylalanine decarboxylase, enolase, and lactate dehydrogenase was monitored during complement-mediated lysis. Three antisera were used: anti-5'-nucleotidase, anti-Chol-1, and anti-rat cerebrum. Anti-5'-nucleotidase serum did not cause the release of any labelled transmitter or of any of the enzymes studied. Anti-Chol-1 serum released choline acetyltransferase and small amounts of enolase and lactate dehydrogenase. Anti-rat cerebrum caused the release of all seven transmitters, choline acetyltransferase, and small amounts of the other three enzymes. It was concluded that 5'-nucleotidase was not present on any of the terminals studied, and that Chol-1 is only present on cholinergic terminals.
从大鼠皮质和海马制备的神经末梢被加载了七种放射性的假定神经递质（血清素、去甲肾上腺素、多巴胺、γ-氨基丁酸、天冬氨酸、谷氨酸和牛磺酸）。在补体介导的裂解过程中监测这些递质、胆碱乙酰转移酶、3,4-二羟基苯丙氨酸脱羧酶、烯醇化酶和乳酸脱氢酶的释放。使用了三种抗血清：抗5'-核苷酸酶、抗Chol-1和抗大鼠大脑。抗5'-核苷酸酶血清未导致任何标记递质或所研究的任何一种酶的释放。抗Chol-1血清释放了胆碱乙酰转移酶以及少量的烯醇化酶和乳酸脱氢酶。抗大鼠大脑导致所有七种递质、胆碱乙酰转移酶以及少量其他三种酶的释放。得出的结论是，在所研究的任何末梢上均不存在5'-核苷酸酶，并且Chol-1仅存在于胆碱能末梢上。
