化学诱导甲状腺功能减退时大鼠脑六个区域腺苷代谢酶活性的变化
Changes in the activities of adenosine-metabolizing enzymes in six regions of the rat brain on chemical induction of hypothyroidism.
作者信息
Mazurkiewicz D, Saggerson D
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1989 Jul 15;261(2):667-72. doi: 10.1042/bj2610667.
Abstract
- Rats (4 weeks old) were made hypothyroid by treatment with propylthiouracil and a low-iodine diet for a further period of 4 weeks. Synaptosomal membranes, myelin and 105,000 g soluble fractions were obtained from six regions of the brain. 2. Hypothyroidism resulted in 2-5-fold increases in membrane-bound 5'-nucleotidase activity in synaptosomal fractions obtained from cerebellum, cortex, striatum and hippocampus. By contrast, myelin 5'-nucleotidase activity was slightly increased only in the medulla oblongata. 3. Hypothyroidism did not change adenosine deaminase activity, but decreased adenosine kinase activity by approx. 40% in soluble fractions obtained from cerebellum, hippocampus, striatum and hypothalamus. 4. It is suggested that these changes in hypothyroidism, in particular the increases in 5'-nucleotidase activity, could enhance the neuromodulatory effect of adenosine to decrease neurotransmitter release.
摘要
- 4周龄大鼠通过丙硫氧嘧啶治疗和低碘饮食进一步处理4周后造成甲状腺功能减退。从大脑的六个区域获取突触体膜、髓磷脂和105,000g可溶性组分。2. 甲状腺功能减退导致从小脑、皮质、纹状体和海马体获得的突触体组分中膜结合5'-核苷酸酶活性增加2至5倍。相比之下，仅延髓中的髓磷脂5'-核苷酸酶活性略有增加。3. 甲状腺功能减退未改变腺苷脱氨酶活性，但使从小脑、海马体、纹状体和下丘脑获得的可溶性组分中的腺苷激酶活性降低约40%。4. 有人提出，甲状腺功能减退中的这些变化，特别是5'-核苷酸酶活性的增加，可能增强腺苷的神经调节作用以减少神经递质释放。
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