Chirieri-Kovacs E, Vasilescu V
Physiol Chem Phys. 1982;14(3):281-9.
Retina is an excitable system containing approximately 90% water. As we found that deuteration selectively changes amplitudes and latencies of retina biopotentials, specifically the ON and OFF responses, we used it to probe the role of water in those processes. A study of the retina deuteration kinetics was simultaneously performed. This revealed the existence of at least two retinal water compartments. The data suggested a third compartment also, with a lower motional "degree of freedom," existing where H2O-D2O exchange becomes important only after saturation by D2O of the first two compartments. Correlation of the electrophysiological effects of D2O with the kinetic data suggests that the ON response is related to the first water compartment and the OFF response to the third. The results point to independence on the ON and OFF response mechanisms and, very probably, to their different morphological origins.
视网膜是一个约含90%水分的可兴奋系统。由于我们发现氘化选择性地改变了视网膜生物电位的幅度和潜伏期，特别是开反应和关反应，我们用它来探究水在这些过程中的作用。同时进行了一项关于视网膜氘化动力学的研究。这揭示了至少存在两个视网膜水室。数据还表明存在第三个水室，其运动“自由度”较低，只有在前两个水室被D2O饱和后，H2O-D2O交换才变得重要。D2O的电生理效应与动力学数据的相关性表明，开反应与第一个水室有关，关反应与第三个水室有关。结果表明开反应和关反应机制相互独立，而且很可能它们具有不同的形态学起源。
