Lobyshev V I, Vishnevskaia Z I
Biofizika. 1984 Mar-Apr;29(2):184-8.
An analysis was carried out of Gross-Butler type equations describing relationship between deuterium isotope effect in reactions with proton transfer and deuterium concentration in the medium. It has been shown that with all possible coefficient values of isotopic fractionation of enzyme functional group protons the shape of indicated relationships qualitatively differs from the experimentally observed one for myosin hydrolysis of ATP. This discrepancy as well as uniform nonlinear change of myosin denaturation temperature and of kinetic isotope effect in myosin hydrolysis of ATP on deuterium fraction in solution give evidence of D2O effect as a solvent and point to an essential role of conformational - dynamic processes in the course of enzymic catalysis with myosin.
对描述质子转移反应中氘同位素效应与介质中氘浓度之间关系的格罗斯 - 巴特勒型方程进行了分析。结果表明，对于酶官能团质子的所有可能同位素分馏系数值，所指示关系的形状在质量上与实验观察到的肌球蛋白水解ATP的关系不同。这种差异以及肌球蛋白变性温度的均匀非线性变化和肌球蛋白水解ATP时动力学同位素效应随溶液中氘分数的变化，证明了D2O作为溶剂的效应，并指出了构象 - 动态过程在肌球蛋白酶催化过程中的重要作用。
