[SV40病毒基因组与酵母载体YEp 13的同时克隆]
[Cloning of the SV40 virus genome simultaneously with yeast vector YEp 13].
作者信息
Granovskiĭ N N, Galkina G M, Manykin A A, Zhdanov V M
出版信息
Vopr Med Khim. 1983 May-Jun;29(3):11-4.
PMID:6308901
Abstract
Recombinant plasmides were constructed, which contained all the genome of virus SV 40 and were able to replicate in bacterial and yeast cells. The plasmides were dissimilar in the orientation of the virus SV 40 genome towards the vector YEp 13. Reversed correlation was found between molecular weight and the transformating activity of plasmides for yeast cells.
摘要
构建了重组质粒，其包含病毒SV 40的所有基因组，并且能够在细菌和酵母细胞中复制。这些质粒在病毒SV 40基因组相对于载体YEp 13的方向上有所不同。发现质粒的分子量与对酵母细胞的转化活性之间呈负相关。
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