Bösterling B, Trudell J R
Biochem Biophys Res Commun. 1983 Jul 29;114(2):850-4. doi: 10.1016/0006-291x(83)90859-8.
Leukotriene B4 (LTB) was found to be metabolized by suspensions of rat liver microsomes in the presence of NADPH and oxygen. The rate of LTB metabolism was also measured in reconstituted systems of both micelles and phospholipid vesicles containing cytochrome P-450-LM2, NADPH cytochrome P-450 reductase, and cytochrome b5. A 1 microM concentration of LTB was metabolized by rat hepatic microsomes at a rate of 4 pmol LTB/min/nmole P-450, and by vesicle and micelle reconstituted systems at 3 pmole/min/nmole P-450-LM2. At this rate a 10 g rat liver exposed to 1 microM LTB can metabolize 30 micrograms per hour. In that the leukotrienes are pharmacologically active at nanomolar concentrations, hepatic metabolism may be an important pathway of leukotriene inactivation.
在存在还原型辅酶Ⅱ（NADPH）和氧气的情况下，发现白三烯B4（LTB）可被大鼠肝脏微粒体悬液代谢。在含有细胞色素P - 450 - LM2、NADPH细胞色素P - 450还原酶和细胞色素b5的胶束和磷脂囊泡重构系统中也测定了LTB的代谢速率。1微摩尔浓度的LTB被大鼠肝脏微粒体以4皮摩尔LTB/分钟/纳摩尔P - 450的速率代谢，被囊泡和胶束重构系统以3皮摩尔/分钟/纳摩尔P - 450 - LM2的速率代谢。以此速率，一只10克重的大鼠肝脏暴露于1微摩尔LTB时每小时可代谢30微克。鉴于白三烯在纳摩尔浓度下具有药理活性，肝脏代谢可能是白三烯失活的重要途径。
