Mendelsohn J, Trowbridge I, Castagnola J
Blood. 1983 Oct;62(4):821-6.
A monoclonal antibody, 42/6, which blocks the binding of transferrin to its receptor on the cell membrane, inhibits proliferation of human lymphocytes stimulated by phytohemagglutinin. Anti-receptor antibody B3/25, which does not block transferrin binding, does not alter the mitogenic response. Addition of soluble iron, in the form of ferric nitrilotriacetic acid, results in partial reversal of inhibition. Lymphocytes in the quiescent phase of the cell cycle at the time of 42/6 antibody addition are unable to traverse S phase, whereas cells actively proliferating when antibody is added are sensitive to its inhibitory effects throughout all phases of the cell cycle. Inhibition is static rather than cidal, since it can be reversed by removal of antibody after up to 48 hr of exposure.
一种单克隆抗体42/6，可阻断转铁蛋白与其在细胞膜上的受体的结合，抑制由植物血凝素刺激的人淋巴细胞增殖。抗受体抗体B3/25，不阻断转铁蛋白结合，不改变促有丝分裂反应。以次氮基三乙酸铁的形式添加可溶性铁，可导致抑制作用部分逆转。在添加42/6抗体时处于细胞周期静止期的淋巴细胞无法穿越S期，而在添加抗体时活跃增殖的细胞在细胞周期的所有阶段均对其抑制作用敏感。抑制是静态而非杀伤性的，因为在长达48小时的暴露后去除抗体可使其逆转。
