Hayashi M, Sornberger G C, Huber G L
Chest. 1978 Apr;73(4):515-8. doi: 10.1378/chest.73.4.515.
Histochemical quantification of goblet cells and measurements of the epithelial thickness were made in the trachea of male and female rats exposed to fresh whole tobacco smoke for 30 consecutive days. Exposure to tobacco smoke resulted in an increase in the total goblet cell count in both sexes, with the female rats differentially responding to a greater degree than the male rats. There was a shift from PAS-positive to alcian blue-positive goblet cells, an increase in the number of both "large" and "small" goblet cells, and an increase in the epithelial thickness. PAS-positive "small" cells, the major glycoprotein-reactive cell in the rat tracheal epithelium, increased in the female rats but showed no increase in the male rats. On the other hand, male rats contained a substantially higher proportion of alcian blue-positive cells than did female rats, both before and after exposure to the smoke. The significance of these observations in relation to the prevalence of chronic bronchitis in human smokers is discussed.
对连续30天暴露于新鲜全烟草烟雾中的雄性和雌性大鼠的气管进行杯状细胞的组织化学定量分析和上皮厚度测量。暴露于烟草烟雾导致两性的杯状细胞总数增加，雌性大鼠的反应程度比雄性大鼠更大。杯状细胞出现从PAS阳性向阿尔辛蓝阳性的转变，“大”和“小”杯状细胞数量均增加，上皮厚度也增加。PAS阳性的“小”细胞是大鼠气管上皮中主要的糖蛋白反应性细胞，在雌性大鼠中增加，但在雄性大鼠中未增加。另一方面，无论在暴露于烟雾之前还是之后，雄性大鼠中阿尔辛蓝阳性细胞的比例都显著高于雌性大鼠。讨论了这些观察结果与人类吸烟者慢性支气管炎患病率的关系。
