Shukla S D, Buxton D B, Olson M S, Hanahan D J
J Biol Chem. 1983 Sep 10;258(17):10212-4.
Acetylglyceryl ether phosphorylcholine (AGEPC), or 1-O-Alkyl-2-acetyl-sn-glyceryl 3-phosphorylcholine, has been shown to have a dramatic influence on phosphoinositide metabolism in isolated rat hepatocytes and upon glycogenolysis in the intact perfused rat liver. Addition of 5 X 10(-10) M AGEPC to 32Pi-labeled rat hepatocytes resulted in up to a 30 to 40% decrease in the [32Pi]phosphatidylinositol 4,5-bisphosphate within 10 s. The 32P content of phosphatidylinositol 4-phosphate decreased approximately 25% within 60 s, while a 5 to 8% decrease in [32P]phosphatidylinositol was observed only after 2 to 5 min of incubation of hepatocytes with AGEPC. Infusion of AGEPC (2 X 10(-10) M) into perfused livers resulted in a 3-fold increase in the glucose output in the effluent perfusate within 2 min. Interestingly, when a 500-fold higher concentration, i.e. 1 X 10(-7) M, of 1-O-alkyl-sn-glyceryl 3-phosphorylcholine or the stereoisomer 3-O-alkyl-2-acetyl-sn-glyceryl 1-phosphorylcholine was infused, no increase in the hepatic glucose output was seen. These observations lead to the conclusion that AGEPC exerts a potent influence on the polyphosphoinositide metabolism and glycogenolysis in rat liver and establishes the liver as an ideal system in which to conduct a detailed inquiry into the biochemical mechanism(s) responsible for the biological action of this unusual phospholipid.
乙酰甘油醚磷酸胆碱（AGEPC），即1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱，已被证明对分离的大鼠肝细胞中的磷酸肌醇代谢以及完整灌注的大鼠肝脏中的糖原分解有显著影响。向用32Pi标记的大鼠肝细胞中添加5×10(-10) M的AGEPC，在10秒内[32Pi]磷脂酰肌醇4,5-二磷酸最多可减少30%至40%。磷脂酰肌醇4-磷酸的32P含量在60秒内大约降低25%，而只有在肝细胞与AGEPC孵育2至5分钟后，才观察到[32P]磷脂酰肌醇减少5%至8%。向灌注肝脏中注入AGEPC（2×10(-10) M），在2分钟内流出灌注液中的葡萄糖输出增加了3倍。有趣的是，当注入浓度高500倍，即1×10(-7) M的1-O-烷基-sn-甘油-3-磷酸胆碱或立体异构体3-O-烷基-2-乙酰基-sn-甘油-1-磷酸胆碱时，未观察到肝脏葡萄糖输出增加。这些观察结果得出结论，AGEPC对大鼠肝脏中的多磷酸肌醇代谢和糖原分解有强大影响，并确立了肝脏是一个理想的系统，可在其中详细探究负责这种不寻常磷脂生物作用的生化机制。
