Cameron I L, Dung H C, Hunter K E, Hazlewood C F
J Cell Physiol. 1983 Sep;116(3):409-14. doi: 10.1002/jcp.1041160320.
Erythrocyte populations from newborn and mature mice were characterized according to: size; ultrastructural features; water content; concentration of intraerythrocyte elements including Na, Cl, K, P, S, Mg, and Fe; and the spinlattice (T1) and spin-spin relaxation times of water protons as measured by nuclear magnetic resonance (NMR) spectroscopy. A significant increase in the T2 time from 142 +/- 3 msec to 184 +/- 3 msec occurred during erythrocyte maturation. This change in T2 time was correlated with a change from a polyribosome-rich hemoglobin-poor cell type to a polyribosome-absent hemoglobin-rich cell type. The change in T2 time could also be correlated to a significantly higher K and P concentration in the mature erythrocytes. The change in T2 time was not correlated to a change in cellular water content or to the concentration of any of the other elements measured by electron probe X-ray microanalysis. If the NMR relaxation times of water molecules truly reflect their average motional freedom, then the findings suggest that greater water ordering interaction occurs in the ribosome containing immature erythrocyte.
大小；超微结构特征；含水量；红细胞内元素（包括钠、氯、钾、磷、硫、镁和铁）的浓度；以及通过核磁共振（NMR）光谱测量的水质子的自旋晶格（T1）和自旋 - 自旋弛豫时间。在红细胞成熟过程中，T2时间从142±3毫秒显著增加到184±3毫秒。T2时间的这种变化与从富含多核糖体、血红蛋白含量低的细胞类型向缺乏多核糖体、血红蛋白含量高的细胞类型的转变相关。T2时间的变化也可能与成熟红细胞中显著更高的钾和磷浓度相关。T2时间的变化与细胞含水量的变化或通过电子探针X射线微分析测量的任何其他元素的浓度均无关联。如果水分子的NMR弛豫时间真的反映了它们的平均运动自由度，那么这些发现表明在含有核糖体的未成熟红细胞中存在更强的水有序相互作用。
