Matsui K, Higashi K, Miyazaki K, Fukunaga K, Maeyama M, Miyamoto E
Jpn J Pharmacol. 1983 Apr;33(2):385-93. doi: 10.1254/jjp.33.385.
We report on hormonal regulation of protein kinases in the rabbit myometrium. The injection of estrogen or progesterone produced 5.2 +/- 0.7- or 4.7 +/- 0.4-fold increases, respectively, of the total activity when histone was used as the substrate in the cytosol fraction of the tissue. The analysis of the activity by DEAE-cellulose column chromatography revealed that estrogen had no effect on increases in the levels in both types I and II cAMP-dependent protein kinases, on the basis of mg protein, while progesterone produced a 50% decrease in type I cAMP-dependent protein kinase and a 50% increase in type II cAMP-dependent protein kinase. Thus, the activity ratio of types II to type I did not change by treatment with estrogen and increased from 2.8 to 7.9 with progesterone administration. As the activity patterns by treatment with human chorionic gonadotropin closely resembled those seen with progesterone, our findings in case of the latter hormone are probably valid. The results indicate that the levels of protein kinases in the myometrium are altered in response to estrogen and progesterone and may possibly be involved in the physiological functions of the tissue.
我们报告了兔子宫肌层中蛋白激酶的激素调节情况。当以组蛋白作为底物时，在组织的胞质溶胶部分，注射雌激素或孕酮分别使总活性增加了5.2±0.7倍或4.7±0.4倍。通过DEAE - 纤维素柱色谱法对活性进行分析表明，基于每毫克蛋白质，雌激素对I型和II型环磷酸腺苷（cAMP）依赖性蛋白激酶的水平增加均无影响，而孕酮使I型cAMP依赖性蛋白激酶减少50%，使II型cAMP依赖性蛋白激酶增加50%。因此，II型与I型的活性比在雌激素处理后未改变，而在给予孕酮后从2.8增加到7.9。由于人绒毛膜促性腺激素处理后的活性模式与孕酮处理后的非常相似，我们关于后一种激素的研究结果可能是有效的。结果表明，子宫肌层中蛋白激酶的水平会因雌激素和孕酮而改变，并且可能参与了该组织的生理功能。
