Nouri L A, Sordelli D O, Cerquetti M C, Saavedra J M, Hooke A M, Bellanti J A
Pediatr Res. 1983 Aug;17(8):657-61. doi: 10.1203/00006450-198308000-00010.
Pulmonary clearance of Staphylococcus aureus and plasma angiotensin-converting enzyme (ACE) activity were investigated in an experimental mouse model of kerosene aspiration. Twenty-four hours after acute kerosene aspiration, mice were exposed for 30 min to an aerosol containing the pathogen, and the uncleared bacteria ratio (UBR) determined 4 h after nebulization. The results showed a significant increase (P = 0.004) in UBR in animals with severe pneumonitis (0.44 +/- 0.05) when compared with controls (0.24 +/- 0.03). This impairment in lung clearance correlated with the increase in lung weight and the decrease in plasma ACE levels. Mice with kerosene pneumonitis had zones of lung injury, and areas with no gross signs of tissue damage. Lung clearance of S. aureus was significantly impaired in damaged areas whereas it was no different from controls in the non-affected areas. It is suggested that the measurement of plasma ACE activity may be an adjunct in the assessment of the extent of lung injury in hydrocarbon aspiration in children.
在煤油吸入的实验小鼠模型中，研究了金黄色葡萄球菌的肺清除率和血浆血管紧张素转换酶（ACE）活性。急性煤油吸入24小时后，将小鼠暴露于含有病原体的气溶胶中30分钟，并在雾化后4小时测定未清除细菌比率（UBR）。结果显示，与对照组（0.24±0.03）相比，患有严重肺炎的动物（0.44±0.05）的UBR显著增加（P = 0.004）。肺清除功能的这种损害与肺重量增加和血浆ACE水平降低相关。患有煤油性肺炎的小鼠有肺损伤区域和无明显组织损伤体征的区域。金黄色葡萄球菌在受损区域的肺清除功能明显受损，而在未受影响区域与对照组无差异。提示血浆ACE活性的测定可能有助于评估儿童烃类吸入所致肺损伤的程度。
