离体胰岛中胰岛素分泌和生长抑素受体募集的差异调控
Differential control of insulin secretion and somatostatin-receptor recruitment in isolated pancreatic islets.
作者信息
Sussman K E, Pollard H B, Leitner J W, Nesher R, Adler J, Cerasi E
出版信息
Biochem J. 1983 Jul 15;214(1):225-30. doi: 10.1042/bj2140225.
Abstract
Somatostatin receptors appear to be localized to secretory granules in pancreatic islet homogenates. Recruitment of these receptors to the islet-cell surfaces may mark the contact event between secretory granules and plasma membranes before release of insulin by fission. Isethionate, an impermeant anionic replacement for chloride, blocks the release step but does not affect receptor recruitment. By contrast, low concentrations of phenothiazine drugs, such as trifluoperazine and promethazine, inhibit both receptor recruitment and secretion. Scatchard analysis of phenothiazine effects on somatostatin receptors reveals that these drugs reduce the number of receptors but do not affect the affinity of the receptor for somatostatin. These data indicate that membrane contact and fission steps during exocytosis can be biochemically separated.
摘要
生长抑素受体似乎定位于胰岛匀浆中的分泌颗粒。这些受体募集到胰岛细胞表面可能标志着在胰岛素通过裂变释放之前分泌颗粒与质膜之间的接触事件。羟乙磺酸盐是一种不能透过的氯离子阴离子替代物，它阻断释放步骤，但不影响受体募集。相比之下，低浓度的吩噻嗪类药物，如三氟拉嗪和异丙嗪，既抑制受体募集又抑制分泌。对吩噻嗪类药物对生长抑素受体影响的Scatchard分析表明，这些药物减少了受体数量，但不影响受体对生长抑素的亲和力。这些数据表明，胞吐过程中的膜接触和裂变步骤可以在生化上分离。
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