大鼠多形核白细胞中腺苷生成的机制。
The mechanism of adenosine production in rat polymorphonuclear leucocytes.
作者信息
Worku Y, Newby A C
出版信息
Biochem J. 1983 Aug 15;214(2):325-30. doi: 10.1042/bj2140325.
Abstract
Adenosine production in intact rat polymorphonuclear leucocytes was studied during 2-deoxyglucose-induced ATP catabolism. A cell-free system containing the cytosolic 5'-nucleotidase (EC 3.1.3.5) as the only phosphohydrolase was also studied. The rate of adenosine formation in both intact cells and the cell-free system showed a similar dependence on energy charge (([ATP] + 1/2 [ADP]/([ATP] + [ADP] + [AMP])), being maximal only at values close to 0.8. Sufficient cytosolic 5'-nucleotidase was present in intact cells to explain the observed rate of adenosine formation. We conclude that the cytosolic 5'-nucleotidase is responsible for adenosine production in rat polymorphonuclear leucocytes. This mechanism provides a direct biochemical link between the energy status of a cell and the rate of adenosine formation.
摘要
在2-脱氧葡萄糖诱导的ATP分解代谢过程中，对完整大鼠多形核白细胞中的腺苷生成进行了研究。还研究了一个无细胞系统，该系统含有胞质5'-核苷酸酶（EC 3.1.3.5）作为唯一的磷酸水解酶。完整细胞和无细胞系统中腺苷形成的速率对能量电荷（([ATP] + 1/2 [ADP]/([ATP] + [ADP] + [AMP])）表现出相似的依赖性，仅在接近0.8的值时达到最大值。完整细胞中存在足够的胞质5'-核苷酸酶来解释观察到的腺苷形成速率。我们得出结论，胞质5'-核苷酸酶负责大鼠多形核白细胞中的腺苷生成。这种机制在细胞的能量状态和腺苷形成速率之间提供了直接的生化联系。
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