Gaillard J L, Lubochinsky B, Rigomier D
Biochim Biophys Acta. 1983 Oct 11;753(3):372-80. doi: 10.1016/0005-2760(83)90061-9.
In Bacillus subtilis, the phosphatidate cytidylyltransferase was localized exclusively in the membrane fraction prepared by sucrose density gradient fractionation. A single enzyme could synthesize the two liponucleotides: CDPdiacylglycerol and dCDPdiacylglycerol. Kinetic experiments and isotopic exchange reactions suggested a ping-pong mechanism. Among the nucleosides monophosphate, CMP specifically reduced the synthesis of both liponucleotides. This inhibition was non-competitive and might be involved in regulation of phospholipid synthesis.
在枯草芽孢杆菌中，磷脂酸胞苷转移酶仅定位于通过蔗糖密度梯度分级分离制备的膜部分。单一酶可合成两种脂核苷酸：CDP二酰甘油和dCDP二酰甘油。动力学实验和同位素交换反应表明存在乒乓机制。在单磷酸核苷中，CMP特异性地降低了两种脂核苷酸的合成。这种抑制是非竞争性的，可能参与磷脂合成的调节。
