Carlen P L, Gurevich N, Polc P
Brain Res. 1983 Jul 25;271(2):358-64. doi: 10.1016/0006-8993(83)90302-5.
The water-soluble inhibitory benzodiazepine, midazolam, was applied in low nanomolar concentrations to CA1 hippocampal neurons in vitro, recorded intracellularly. The drug caused a long-lasting hyperpolarization and moderate conductance increase, which persisted with TTX-induced synaptic blockade or with intracellular injection of Cl- ions, but not in zero Ca2+ perfusate. Calcium spikes elicited in the presence of TTX were enhanced by midazolam. It was concluded that these low nanomolar concentrations, which did not enhance GABA actions, inhibited by augmenting Ca2+ mediated K+-conductance.
水溶性抑制性苯二氮䓬类药物咪达唑仑，以低纳摩尔浓度应用于体外培养的海马CA1神经元，并进行细胞内记录。该药物引起持久的超极化和适度的电导增加，在TTX诱导的突触阻断或细胞内注射Cl-离子时这种情况持续存在，但在无Ca2+灌注液中则不然。在TTX存在的情况下引发的钙峰被咪达唑仑增强。得出的结论是，这些不增强GABA作用的低纳摩尔浓度，通过增强Ca2+介导的K+电导发挥抑制作用。
