Menkes D B, Gallager D W, Reinhard J F, Aghajanian G K
Brain Res. 1983 Aug 1;272(1):1-12. doi: 10.1016/0006-8993(83)90358-x.
Electrophysiological and radioligand binding methods were used to characterize noradrenergic denervation supersensitivity at alpha 1-adrenoceptors in rat thalamus. Denervation was accomplished either by intraventricular or intracerebral injection of the catecholamine neurotoxin 6-hydroxydopamine (6-OHDA). In the physiological studies, the sensitivity of single lateral geniculate neurons to norepinephrine, carbachol, and serotonin was compared in sham and lesioned animals various times after 6-OHDA. Conducted in parallel were radioligand binding studies in which the density and affinity of thalamic alpha 1-adrenoceptors were measured with the specific antagonist [3H]prazosin. The results indicate that denervation produces a selective increase in the sensitivity of geniculate neurons to alpha 1-adrenergic stimulation and a concomitant increase in alpha 1-adrenoceptor density and agonist affinity.
采用电生理和放射性配体结合方法，对大鼠丘脑α1 -肾上腺素能受体去甲肾上腺素能失神经超敏反应进行表征。通过脑室内或脑内注射儿茶酚胺神经毒素6 -羟基多巴胺（6 - OHDA）实现去神经支配。在生理学研究中，比较了假手术组和6 - OHDA处理后不同时间的损伤动物中，单个外侧膝状体神经元对去甲肾上腺素、卡巴胆碱和5 -羟色胺的敏感性。同时进行了放射性配体结合研究，用特异性拮抗剂[3H]哌唑嗪测量丘脑α1 -肾上腺素能受体的密度和亲和力。结果表明，去神经支配导致膝状体神经元对α1 -肾上腺素能刺激的敏感性选择性增加，同时α1 -肾上腺素能受体密度和激动剂亲和力也增加。
