Martin X, Dolivo M
Brain Res. 1983 Aug 29;273(2):253-76. doi: 10.1016/0006-8993(83)90850-8.
The herpes virus suis has been used as a tracer for the pathways in the central nervous system of the rat. The viruses have been inoculated in various peripheral structures innervated by the trigeminal nerve, namely in the cornea by instillation or scarification, in the anterior chamber of the eye by injection and also by subconjunctival injection, nasal instillation and injection in the masseter muscle. The herpes virus suis is easy to detect by immunofluorescence or electron microscopy, the tracing is precise because it does not diffuse, as some other tracers. The virus is replicated at the site of inoculation and at each neuronal relay, thus 'fresh' tracer is continuously brought into the system. The herpes virus suis is transported by retrograde axonal flow. It has been observed in the motor sensory, sympathetic and parasympathetic pathways up to the central nuclei, which demonstrates transneuronal transport. The selectivity of this tracer, applied to the trigeminal pathways, has allowed us to understand the function of the 3 types of neurons present in the trigeminal ganglion, namely to confirm their somatotopy and establish their central projections in the trigeminal system.
猪疱疹病毒已被用作大鼠中枢神经系统通路的示踪剂。病毒已接种于三叉神经支配的各种外周结构，即通过滴注或划痕接种于角膜，通过注射接种于眼前房，也可通过结膜下注射、滴鼻以及注射于咬肌。猪疱疹病毒易于通过免疫荧光或电子显微镜检测，示踪精确，因为它不像其他一些示踪剂那样扩散。病毒在接种部位和每个神经元中继处复制，因此“新鲜”的示踪剂不断进入系统。猪疱疹病毒通过逆行轴浆流运输。在运动感觉、交感和副交感神经通路中直至中枢核均观察到该病毒，这表明了跨神经元运输。将这种示踪剂应用于三叉神经通路的选择性，使我们能够了解三叉神经节中存在的3种类型神经元的功能，即确认它们的躯体定位并确定它们在三叉神经系统中的中枢投射。
