Windmoller R, Lye S J, Challis J R
Can J Physiol Pharmacol. 1983 Jul;61(7):722-8. doi: 10.1139/y83-112.
The effects of continuous estradiol-17 beta (E2) infusion (100 micrograms/24 h and 1 mg/24 h) on spontaneous myometrial activity and uterine responsiveness to oxytocin (OT) and prostaglandin (PG) F2 alpha were measured in nonpregnant sheep. Continuous recordings were made of intrauterine pressure (IUP) and uterine electromyographic (EMG) activity. Continuous E2 infusion resulted in an accentuated pattern of uterine quiescence alternating with periods of high activity lasting 6-8 h. Short (3-10 min) periods of elevated resting IUP and intense EMG activity increased in frequency after E2. E2 also increased the rate of rise of IUP cycles. Within 30 h of commencement of E2 infusion the uterine response to a bolus challenge of OT or PGF2 alpha increased significantly; dose-related responses to OT and PGF2 alpha were measured after 4 days E2 infusion. The response to OT was not abolished by infusing meclofenamic acid. We conclude that: (i) continuous E2 infusion results in a pattern of uterine activity characterized by alternating high activity and quiescence, and increased frequency of occasional rises in basal IUP; (ii) E2 induced increases in OT and PGF2 alpha responses are consistent with other reports that E2 increases OT and PGF2 alpha binding to myometrium; (iii) the E2 effect on OT response is probably independent of enhanced PG synthesis.
在未孕绵羊中，测定了持续输注17β -雌二醇（E2）（100微克/24小时和1毫克/24小时）对子宫肌层自发活动以及子宫对催产素（OT）和前列腺素（PG）F2α反应性的影响。连续记录子宫内压（IUP）和子宫肌电图（EMG）活动。持续输注E2导致子宫出现一种更为明显的静止模式，期间交替出现持续6 - 8小时的高活动期。E2处理后，静息IUP短暂升高（3 - 10分钟）和强烈EMG活动的频率增加。E2还增加了IUP周期的上升速率。在开始输注E2的30小时内，子宫对OT或PGF2α推注刺激的反应显著增强；在输注E2 4天后测定了对OT和PGF2α的剂量相关反应。输注甲氯芬那酸并未消除对OT的反应。我们得出以下结论：（i）持续输注E2导致子宫活动模式的特征为高活动期和静止期交替出现，且基础IUP偶尔升高的频率增加；（ii）E2诱导的对OT和PGF2α反应的增强与其他报道一致，即E2增加OT和PGF2α与子宫肌层的结合；（iii）E2对OT反应的影响可能独立于PG合成的增强。
