Gaber B P, Yager P, Sheridan J P, Chang E L
FEBS Lett. 1983 Mar 21;153(2):285-8. doi: 10.1016/0014-5793(83)80625-5.
Hemoglobin has been encapsulated in phospholipid vesicles by extrusion of hemoglobin/lipid mixtures through polycarbonate membranes. This technique avoids the use of organic solvents, sonication, and detergents which have proven deleterious to hemoglobin. The vesicles are homogeneous, with a mean size of 2400 A as determined by photon correlation spectroscopy. The encapsulated hemoglobin binds oxygen reversibly and the vesicles are impermeable to ionic compounds. Hemoglobin encapsulated in egg phosphatidylcholine vesicles converts to methemoglobin within 2 days at 4 degrees C. By contrast, when a mixture of dimyristoyl phosphatidylcholine, cholesterol and dicetyl phosphate is used there is no acceleration in methemoglobin formation, and the preparation is stable for at least 14 days at 4 degrees C.
通过将血红蛋白/脂质混合物挤压通过聚碳酸酯膜，血红蛋白已被包裹在磷脂囊泡中。该技术避免了使用已证明对血红蛋白有害的有机溶剂、超声处理和去污剂。囊泡是均匀的，通过光子相关光谱法测定其平均大小为2400埃。包裹的血红蛋白可逆地结合氧气，并且囊泡对离子化合物是不可渗透的。包裹在鸡蛋磷脂酰胆碱囊泡中的血红蛋白在4℃下2天内会转化为高铁血红蛋白。相比之下，当使用二肉豆蔻酰磷脂酰胆碱、胆固醇和磷酸二鲸蜡酯的混合物时，高铁血红蛋白的形成没有加速，并且该制剂在4℃下至少可稳定14天。
