Hendrich S, Bjeldanes L F
Food Chem Toxicol. 1983 Aug;21(4):479-86. doi: 10.1016/0278-6915(83)90106-0.
Male C57B16 mice were fed on diets containing either 20% cabbage, 20% Brussels sprouts, 20% alfalfa, 5% Schizandra chinensis or 5% Illicium verum (two Chinese medicinal herbs) or on a chow or purified basal diet for 14 days after a 1-wk equilibration period on the basal diet. Liver microsomal fractions were assayed for cytochrome P-450 content, aryl hydrocarbon hydroxylase (AHH) and epoxide hydrolase (EH). Liver microsome-mediated benzo[a]pyrene (BP) metabolism (with and without an EH inhibitor, 1,2-epoxy-3,3,3-trichloropropane) was analysed by HPLC. Liver weights of the animals fed on Brussels sprouts and I. verum were significantly increased compared with those of the animals fed on basal diets. S. chinensis induced a 3-fold increase in cytochrome P-450 (P less than 0.05). Although P-450 induction in the other groups was as high as 1.8-fold (for chow), statistical significance was not established. Chow induced AHH activity 2.2-fold (P less than 0.05), while S. chinensis and alfalfa induced 1.6-fold and 1.7-fold increases, respectively, in AHH activity, although neither increase was statistically significant. EH was stimulated significantly in the following order: I. verum (2.1-fold) greater than chow (1.7-fold) greater than S. chinensis (1.6-fold) greater than Brussels sprouts (1.4-fold). Total levels of BP metabolism and phenol II (primarily 3-hydroxybenzo[a]pyrene) formation were closely associated for each dietary treatment. Total BP metabolism was significantly increased (2.1-fold) in the chow-fed group and increased 1.6-fold in the S. chinensis group (P greater than 0.05). No increase was seen with the other diets. Phenol II formation relative to total metabolites was significantly increased for the S. chinensis and I. verum groups compared to the basal group. Diet-related variations in phenol production relative to total metabolism were eliminated by addition of the EH inhibitor to the incubation media.
雄性C57B16小鼠在基础饮食上经过1周的平衡期后，分别喂食含20%卷心菜、20%抱子甘蓝、20%苜蓿、5%五味子或5%八角（两种中药材）的日粮，或普通饲料或纯化基础日粮，持续14天。对肝微粒体部分进行细胞色素P - 450含量、芳烃羟化酶（AHH）和环氧化物水解酶（EH）的检测。通过高效液相色谱法分析肝微粒体介导的苯并[a]芘（BP）代谢（有和没有EH抑制剂1,2 - 环氧 - 3,3,3 - 三氯丙烷的情况下）。与喂食基础日粮的动物相比，喂食抱子甘蓝和八角的动物肝脏重量显著增加。五味子使细胞色素P - 450增加了3倍（P < 0.05）。虽然其他组的P - 450诱导率高达1.8倍（对于普通饲料），但未达到统计学显著性。普通饲料使AHH活性增加了2.2倍（P < 0.05），而五味子和苜蓿分别使AHH活性增加了1.6倍和1.7倍，尽管这两种增加均无统计学显著性。EH受到刺激的顺序如下：八角（2.1倍）>普通饲料（1.7倍）>五味子（1.6倍）>抱子甘蓝（1.4倍）。每种饮食处理中BP代谢总量和苯酚II（主要是3 - 羟基苯并[a]芘）的形成密切相关。普通饲料喂养组的BP代谢总量显著增加（2.1倍），五味子组增加了1.6倍（P > 0.05）。其他日粮组未见增加。与基础组相比，五味子和八角组相对于总代谢物的苯酚II形成显著增加。通过在孵育培养基中添加EH抑制剂，消除了与饮食相关的相对于总代谢的苯酚产生差异。
