一种用于蛋白质羧基的可裂解交联反应。
A cleavable cross-linking reaction for protein carboxyl groups.
作者信息
Renthal R
出版信息
Int J Pept Protein Res. 1983 Aug;22(2):144-7. doi: 10.1111/j.1399-3011.1983.tb02078.x.
Abstract
L-(+)-tartaric acid dihydrazide was coupled to the purple membrane from Halobacterium halobium with a water soluble carbodiimide. Gel electrophoresis analysis of the product revealed the formation of bacteriorhodopsin dimers, trimers and higher polymers. Most of the cross-links were removed by treatment with papain, demonstrating involvement of the carboxyl-rich carboxyl-terminal region of bacteriorhodopsin in the reaction. The cross-links were cleavable by periodate oxidation.
摘要
L-(+)-酒石酸二酰肼通过水溶性碳二亚胺与嗜盐菌紫膜偶联。对产物进行的凝胶电泳分析显示形成了细菌视紫红质二聚体、三聚体及更高聚物。用木瓜蛋白酶处理可去除大部分交联键，这表明细菌视紫红质富含羧基的羧基末端区域参与了该反应。这些交联键可通过高碘酸盐氧化裂解。
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A cleavable cross-linking reaction for protein carboxyl groups.一种用于蛋白质羧基的可裂解交联反应。
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