Kasprzak A A, Steenkamp D J
J Bacteriol. 1983 Oct;156(1):348-53. doi: 10.1128/jb.156.1.348-353.1983.
The localization of prominent proteins in intact cells of two methylotrophic bacteria, Hyphomicrobium sp. strain X and bacterium W3A1, was investigated by radiochemical labeling with [14C]isethionyl acetimidate. In bacterium W3A1, trimethylamine dehydrogenase was not labeled by the reagent and is, therefore, an intracellular protein, whereas the periplasmic location of the methylamine and methanol dehydrogenases was evidenced by being readily labeled in intact cells. Similarly, an intracellular location of the trimethylamine and dimethylamine dehydrogenases in Hyphomicrobium sp. strain X was indicated, whereas methanol dehydrogenase was periplasmic.
利用[14C]羟乙磺酰乙脒进行放射化学标记，研究了两种甲基营养菌——生丝微菌属X菌株和W3A1菌完整细胞中显著蛋白质的定位。在W3A1菌中，三甲胺脱氢酶未被该试剂标记，因此是一种细胞内蛋白质，而甲胺和甲醇脱氢酶位于周质，这可通过完整细胞中它们易于被标记得到证明。同样，生丝微菌属X菌株中的三甲胺和二甲胺脱氢酶定位于细胞内，而甲醇脱氢酶位于周质。
