用于检测爱泼斯坦-巴尔病毒诱导的抗原和抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for the detection of Epstein-Barr virus-induced antigens and antibodies.
作者信息
Sternås L, Luka J, Kallin B, Rosén A, Henle W, Henle G, Klein G
出版信息
J Immunol Methods. 1983 Oct 14;63(2):171-85. doi: 10.1016/0022-1759(83)90422-2.
Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA- cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.
开发了两种爱泼斯坦-巴尔病毒（EBV）特异性酶联免疫吸附测定（ELISA）试验。一种基于使用在阿糖胞苷存在下高度诱导的病毒生产细胞的粗提取物（提供EA + VCA-细胞）或在无药物情况下（提供EA + VCA +细胞），适用于检测针对与裂解病毒周期相关的抗原复合物的抗体；即EA、VCA，可能还有MA。第二种试验使用纯化的EBNA，可用于检测针对转化相关核抗原的抗体。预计这些试验将应用于剖析患者对各种抗原亚成分的抗体反应、生化纯化过程中对EBV编码抗原的监测以及筛选杂交瘤培养物的用过培养基中的EBV特异性抗体。
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