用过氧化物酶标记的特异性免疫球蛋白检测爱泼斯坦-巴尔病毒抗原和抗体。

Detection of Epstein-Barr virus antigens and antibodies by peroxidase-labeled specific immunoglobulins.

作者信息

Kurstak E, de Thé G, van den Hurk J, Charpentier G, Kurstak C, Tijssen P, Morisset R

出版信息

J Med Virol. 1978;2(3):189-200. doi: 10.1002/jmv.1890020302.

DOI:10.1002/jmv.1890020302

Abstract

Detection of the Epstein-Barr (EBV) antigens, early antigen (EA), viral capsid antigen (VCA), and nuclear antigen (EBNA) by the indirect immunoperoxidase technique was highly sensitive. Antibody titers to EBNA, EA, and VCA were determined in more than 25 sera of patients with Burkitt lymphoma (BL), nasopharyngeal carcinoma (NPC), or normal persons. A good correlation between the titers of these antigens was obtained by the immunoperoxidase and immunofluorescence methods. The indirect (anti-IgG) immunoperoxidase technique for the detection of EBNA is, in contrast to the indirect immunofluorescence method, highly sensitive. EBNA was associated with the chromosomes in cells arrested in the metaphase with colchicine.

摘要

采用间接免疫过氧化物酶技术检测爱泼斯坦-巴尔病毒（EBV）抗原、早期抗原（EA）、病毒衣壳抗原（VCA）和核抗原（EBNA）具有高度敏感性。测定了25例以上伯基特淋巴瘤（BL）、鼻咽癌（NPC）患者或正常人血清中针对EBNA、EA和VCA的抗体滴度。通过免疫过氧化物酶法和免疫荧光法，这些抗原的滴度之间具有良好的相关性。与间接免疫荧光法相比，用于检测EBNA的间接（抗IgG）免疫过氧化物酶技术具有高度敏感性。EBNA与用秋水仙碱使细胞阻滞在中期时的染色体相关。