人类胰岛素瘤中BK病毒变体的游离DNA

Episomal DNA of a BK virus variant in a human insulinoma.

作者信息

Caputo A, Corallini A, Grossi M P, Carrà L, Balboni P G, Negrini M, Milanesi G, Federspil G, Barbanti-Brodano G

出版信息

J Med Virol. 1983;12(1):37-49. doi: 10.1002/jmv.1890120105.

DOI:10.1002/jmv.1890120105

PMID:6311966

Abstract

BK virus (BKV) DNA was detected by blot hybridization in a human adenoma of pancreatic islets from patient I.R. BKV DNA was free, and no evidence was found of viral sequences integrated into cellular DNA. Virus was rescued by transfection of human embryonic fibroblasts with tumor DNA. The DNA from rescued virus (BKV-IR) was different from wild-type BKV DNA by restriction endonuclease mapping. The genome of BKV-IR is 235 base pairs (bp) shorter than the genome of wild-type BKV. This alteration originates from a deletion of approximately 300 bp involving HindIII fragments B and D, and an insertion of 70 bp in the region of HindIII fragment C. Transformation of hamster kidney cells was induced by total tumor DNA as well as by BKV-IR and BKV-IR DNA. No antibodies to BKV tumor (T) antigen were detected in the patient's serum by immunofluorescence. The significance of episomal BKV DNA in a human tumor is discussed.

摘要

通过印迹杂交在患者I.R.的胰岛人腺瘤中检测到BK病毒（BKV）DNA。BKV DNA是游离的，未发现病毒序列整合到细胞DNA中的证据。通过用肿瘤DNA转染人胚胎成纤维细胞拯救出病毒。通过限制性内切酶图谱分析，拯救出的病毒（BKV-IR）的DNA与野生型BKV DNA不同。BKV-IR的基因组比野生型BKV的基因组短235个碱基对（bp）。这种改变源于约300 bp的缺失，涉及HindIII片段B和D，以及在HindIII片段C区域插入70 bp。总肿瘤DNA以及BKV-IR和BKV-IR DNA均可诱导仓鼠肾细胞发生转化。通过免疫荧光在患者血清中未检测到针对BKV肿瘤（T）抗原的抗体。讨论了游离型BKV DNA在人类肿瘤中的意义。