Corallini A, Pagnani M, Viadana P, Silini E, Mottes M, Milanesi G, Gerna G, Vettor R, Trapella G, Silvani V
Int J Cancer. 1987 Jan 15;39(1):60-7. doi: 10.1002/ijc.2910390111.
BK virus (BKV) DNA was detected by Southern blot hybridization in 19 out of 74 (25.6%) human brain tumors and in 4 out of 9 (44.4%) human tumors of pancreatic islets. BKV DNA was free, in an episomal state and generally in a low copy number (0.2 to 2 genome equivalents per cell). Only occasional tumors contained 10 to 20 genome copies per cell. Viral DNA sequences integrated into cellular DNA were not detected. A number of tumors expressed BKV-specific RNA and T antigen. By transfection of total tumor DNA into human embryonic fibroblasts, viruses with the biological and antigenic properties of BKV were rescued from 6 brain tumors and from 2 tumors of pancreatic islets. Restriction endonuclease mapping of the genomes of the rescued viruses showed that they differ from wild-type BKV. They are all similar to each other and to BKV-IR, a virus previously rescued from a human tumor of pancreatic islets, suggesting the possible association of a BKV variant with specific types of human neoplasms. The significance of the relationship of these BKV variants to human tumors and their possible etiologic role in human oncogenesis are discussed.
通过Southern印迹杂交法在74例人脑肿瘤中的19例（25.6%）以及9例人胰岛肿瘤中的4例（44.4%）检测到BK病毒（BKV）DNA。BKV DNA呈游离状态，以附加体形式存在，且通常拷贝数较低（每细胞0.2至2个基因组当量）。仅有少数肿瘤每细胞含有10至20个基因组拷贝。未检测到整合到细胞DNA中的病毒DNA序列。许多肿瘤表达BKV特异性RNA和T抗原。通过将肿瘤总DNA转染到人胚胎成纤维细胞中，从6例脑肿瘤和2例胰岛肿瘤中拯救出具有BKV生物学和抗原特性的病毒。对拯救出的病毒基因组进行限制性内切酶图谱分析表明，它们与野生型BKV不同。它们彼此相似，且与先前从人胰岛肿瘤中拯救出的BKV-IR病毒相似，提示BKV变异体可能与特定类型的人类肿瘤相关。讨论了这些BKV变异体与人类肿瘤的关系及其在人类肿瘤发生中可能的病因学作用。
