Negrini M, Rimessi P, Mantovani C, Sabbioni S, Corallini A, Gerosa M A, Barbanti-Brodano G
Institute of Microbiology, School of Medicine, University of Ferrara, Italy.
J Gen Virol. 1990 Nov;71 ( Pt 11):2731-6. doi: 10.1099/0022-1317-71-11-2731.
Episomal BK virus (BKV) DNA was detected in primary human brain tumours, in Kaposi's sarcoma and in cell lines from brain tumours. Ewing sarcoma and osteogenic sarcoma. Infectious BKV was rescued from several tumours and tumour cell lines by transfection of total cellular DNA into human embryonic fibroblasts. Restriction endonuclease and nucleotide sequence analysis showed that all the rescued viruses are similar to BKV-IR, a BK variant previously isolated from a human tumour of pancreatic islets, indicating that a specific BKV strain may be associated with certain types of human tumours. All the variants contain a putative transposable elements in the regulatory region of the viral genome. This region has mutagenic properties and enhancing activity in transformation, suggesting a possible role of these variants in tumour induction or progression.
在原发性人脑肿瘤、卡波西肉瘤以及脑肿瘤、尤因肉瘤和成骨肉瘤的细胞系中检测到了游离型BK病毒（BKV）DNA。通过将肿瘤细胞的总DNA转染到人胚成纤维细胞中，从多个肿瘤和肿瘤细胞系中拯救出了具有感染性的BKV。限制性内切酶和核苷酸序列分析表明，所有拯救出的病毒均与BKV-IR相似，BKV-IR是先前从一例人胰岛肿瘤中分离出的BK病毒变异株，这表明特定的BKV毒株可能与某些类型的人类肿瘤有关。所有变异株在病毒基因组的调控区域均含有一个推定的转座元件。该区域具有诱变特性且在转化过程中具有增强活性，提示这些变异株在肿瘤诱导或进展中可能发挥作用。
