Trevisan A, Gudat F, Guggenheim R, Bianchi L
Minerva Med. 1983 Sep 22;74(36):2073-80.
In the present work a minibead method has been employed to study human hepatocyte receptors for albumin-coupled latex particles. The cell-latex reaction was observed in both phase contrast and scanning electron microscopy. It has been demonstrated that human hepatocytes exhibit bindings sites for different forms of glutaraldehyde-treated albumin, and the ligand has proved to be species-nonspecific. The albumin binding activity is temperature-dependent and can be only partially blocked by preincubation with free, glutaraldehyde-treated protein, unless hepatocytes are pretreated with vinblastine sulfate. The possible biological role of these receptors as regards the infection due to the hepatitis B virus (that shares common, but not identical binding sites) is also discussed.
在本研究中，采用微珠法研究人肝细胞对白蛋白偶联乳胶颗粒的受体。在相差显微镜和扫描电子显微镜下观察细胞与乳胶的反应。已证明人肝细胞对不同形式的戊二醛处理的白蛋白表现出结合位点，并且该配体已被证明是种属非特异性的。白蛋白结合活性是温度依赖性的，并且只有在肝细胞用硫酸长春碱预处理后，才能通过与游离的、戊二醛处理的蛋白质预孵育而部分被阻断。还讨论了这些受体对于乙型肝炎病毒感染（其具有共同但不完全相同的结合位点）可能的生物学作用。
