Thung S N, Gerber M A
Liver. 1983 Oct;3(5):290-4. doi: 10.1111/j.1600-0676.1983.tb00880.x.
Recent studies of hepatitis B virus suggest that polymeric human serum albumin may facilitate the attachment of the virus via albumin receptors to hepatocytes during the infectious process. If this hypothesis is correct, hepatocytes should express binding sites for polymeric albumin. We employed the red blood cell adherence test using albumin-coated red blood cells as indicator cells on frozen sections of normal human livers to demonstrate these binding sites. Hepatocytes showed binding activity for both polymeric and monomeric albumin from different species. The receptor-ligand interaction was temperature and pH dependent, Ca++ independent and not altered by mercaptoethanol treatment. The binding activity was sensitive to neuraminidase and pronase, but resistant to trypsin, lipase and collagenase digestion. These findings suggest that human hepatocytes display species-non-specific albumin binding sites, which are glycoproteins.
最近对乙型肝炎病毒的研究表明，在感染过程中，聚合人血清白蛋白可能通过白蛋白受体促进病毒与肝细胞的附着。如果这一假设正确，肝细胞应该表达聚合白蛋白的结合位点。我们采用以白蛋白包被的红细胞作为指示细胞的红细胞黏附试验，对正常人肝脏的冰冻切片进行检测，以证明这些结合位点。肝细胞对来自不同物种的聚合白蛋白和单体白蛋白均表现出结合活性。受体 - 配体相互作用依赖于温度和pH值，不依赖于Ca++，且不受巯基乙醇处理的影响。结合活性对神经氨酸酶和链霉蛋白酶敏感，但对胰蛋白酶、脂肪酶和胶原酶消化具有抗性。这些发现表明，人肝细胞具有物种非特异性的白蛋白结合位点，这些位点是糖蛋白。
