Gupta S, Dalela R C, Saxena P K
Toxicol Lett. 1983 Jun;17(1-2):167-73. doi: 10.1016/0378-4274(83)90053-x.
In the present study, effects of phenol (P), 2,4-dinitrophenol (DNP) and pentachlorophenol (PCP) individually, and in three combinations viz., (PCP + DNP)/P (highly antagonistic), (DNP + P)/PCP (additive) and (P + DNP)/PCP (highly synergistic) at three sublethal levels (1/10, 1/15, and 1/20 fractions of 96 h LC50) on 5-nucleotidase activity have been studied in brain, liver, kidney and gills of a freshwater teleost, Notopterus notopterus. The results indicate that 5-nucleotidase activity was inhibited significantly (P less than 0.05; P less than 0.01; P less than 0.001) in these tissues at most of the concentrations after 15 and 30 days of exposure. However, significant stimulation (P less than 0.05) of this enzyme was also notice in kidney at 1/20 of P, and (PCP + DNP)/P combination after 15 days of exposure. Inhibition of this enzyme in tissues possibly represents the uncoupling of oxidative phosphorylation, while stimulation of the enzyme indicates that the concerned phenolic compounds might be inducing the cells of the kidney to produce more of this enzyme.
在本研究中，已在一种淡水硬骨鱼——背眼鱼（Notopterus notopterus）的脑、肝、肾和鳃中，研究了苯酚（P）、2,4-二硝基苯酚（DNP）和五氯苯酚（PCP）单独作用以及三种组合，即（PCP + DNP）/P（高度拮抗）、（DNP + P）/PCP（相加）和（P + DNP）/PCP（高度协同）在三个亚致死水平（96小时半数致死浓度（LC50）的1/10、1/15和1/20）对5-核苷酸酶活性的影响。结果表明，在暴露15天和30天后，这些组织中大多数浓度下的5-核苷酸酶活性均受到显著抑制（P < 0.05；P < 0.01；P < 0.001）。然而，在暴露15天后，在肾脏中，当P为1/20以及（PCP + DNP）/P组合时，也观察到该酶有显著刺激作用（P < 0.05）。组织中该酶的抑制可能代表氧化磷酸化的解偶联，而酶的刺激表明相关酚类化合物可能诱导肾脏细胞产生更多这种酶。
