Nishiyama Y, Tsurumi T, Aoki H, Maeno K
Virology. 1983 Sep;129(2):524-8. doi: 10.1016/0042-6822(83)90195-2.
When human embryonic fibroblasts (HEF) were infected with herpes simplex virus type 2 (HSV-2), replicative viral DNA synthesis and some repair synthesis of cellular DNA were induced at the early stage of infection, but almost all DNA synthesis at the late stage of infection was derived from repair synthesis of cellular and viral DNA (Y. Nishiyama and F. Rapp, Virology 110, 466-475, 1981). In this study, we have assessed the effects of DNA polymerase inhibitors on repair DNA synthesis HSV-2-infected HEF. Both viral and cellular DNA syntheses during the late stage of infection were extremely resistant to aphidicolin and phosphonoacetic acid but partially sensitive to high concentrations of 1-beta-D-arabinofuranosylcytosine, while replicative viral DNA synthesis during the early stage of infection was very sensitive to all of those inhibitors. The results suggest that neither HSV-induced DNA polymerase nor cellular DNA polymerase alpha was involved in the repair synthesis of viral and cellular DNA but that cellular DNA polymerase beta was.
当人胚成纤维细胞（HEF）感染2型单纯疱疹病毒（HSV-2）时，在感染早期可诱导病毒复制性DNA合成以及细胞DNA的一些修复合成，但感染后期几乎所有的DNA合成均源于细胞和病毒DNA的修复合成（Y. Nishiyama和F. Rapp，《病毒学》110，466 - 475，1981）。在本研究中，我们评估了DNA聚合酶抑制剂对HSV-2感染的HEF中修复性DNA合成的影响。感染后期的病毒和细胞DNA合成对阿非迪霉素和膦甲酸具有极强的抗性，但对高浓度的1-β-D-阿拉伯呋喃糖基胞嘧啶部分敏感，而感染早期的病毒复制性DNA合成对所有这些抑制剂都非常敏感。结果表明，HSV诱导的DNA聚合酶和细胞DNA聚合酶α均未参与病毒和细胞DNA的修复合成，而细胞DNA聚合酶β参与了。
