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DNA聚合酶α参与紫外线照射的单纯疱疹病毒的宿主细胞复活。

Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus.

作者信息

Nishiyama Y, Yoshida S, Maeno K

出版信息

J Virol. 1984 Feb;49(2):598-600. doi: 10.1128/JVI.49.2.598-600.1984.

DOI:10.1128/JVI.49.2.598-600.1984
PMID:6319762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255505/
Abstract

Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.

摘要

阿非科林是宿主细胞DNA聚合酶α和单纯疱疹病毒（HSV）诱导的DNA聚合酶的有效抑制剂，但对宿主细胞的DNA聚合酶β和γ没有影响。通过使用HSV的阿非科林抗性突变体（Aphr），研究了DNA聚合酶α在紫外线损伤的HSV的宿主细胞重新激活中的可能作用。每毫升1微克的阿非科林显著抑制了紫外线照射的Aphr形成噬菌斑，这并不影响未照射的Aphr的铺板效率。感染后12小时前添加阿非科林可抑制照射后的Aphr形成噬菌斑，而感染后36小时后其对阿非科林变得不敏感。结果强烈表明宿主细胞DNA聚合酶α参与了紫外线照射的HSV DNA的修复。

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本文引用的文献

1
Characterization of deoxyribonucleic acid repair synthesis in permeable human fibroblasts.可渗透人成纤维细胞中脱氧核糖核酸修复合成的特性分析
Biochemistry. 1982 May 11;21(10):2557-64. doi: 10.1021/bi00539a040.
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Effects of DNA polymerase inhibitors on replicative and repair DNA synthesis in ultraviolet-irradiated HeLa cells.DNA聚合酶抑制剂对紫外线照射的HeLa细胞中复制性和修复性DNA合成的影响。
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Differential responses of log and stationary phase human fibroblasts to inhibition of DNA repair by aphidicolin.对数期和静止期人成纤维细胞对阿非科林抑制DNA修复的不同反应。
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Inhibitors of repair DNA synthesis.DNA修复合成抑制剂。
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Aphidicolin inhibits repair of DNA in UV-irradiated human fibroblasts.阿非科林抑制紫外线照射后的人成纤维细胞中的DNA修复。
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New views of the biochemistry of eucaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase alpha.通过一种不同寻常的DNA聚合酶α抑制剂——阿非科林揭示的真核生物DNA复制生物化学新观点。
Cell. 1981 Mar;23(3):647-8. doi: 10.1016/0092-8674(81)90426-8.
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DNA polymerase alpha mutants from a Drosophila melanogaster cell line.来自黑腹果蝇细胞系的DNA聚合酶α突变体。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7049-53. doi: 10.1073/pnas.77.12.7049.
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Mammalian mutator mutant with an aphidicolin-resistant DNA polymerase alpha.具有阿非科林抗性DNA聚合酶α的哺乳动物突变体。
Proc Natl Acad Sci U S A. 1983 Feb;80(3):797-801. doi: 10.1073/pnas.80.3.797.
9
Identification of DNA polymerase(s) involved in the repair of viral and cellular DNA in herpes simplex virus type 2-infected cells.鉴定参与2型单纯疱疹病毒感染细胞中病毒和细胞DNA修复的DNA聚合酶。
Virology. 1983 Sep;129(2):524-8. doi: 10.1016/0042-6822(83)90195-2.
10
Correlation of increased nuclease activity with enhanced virus reactivation.核酸酶活性增加与病毒再激活增强之间的相关性。
Exp Cell Res. 1982 Apr;138(2):485-9. doi: 10.1016/0014-4827(82)90205-1.

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