Bywater M, Bywater R, Hellman L
Anal Biochem. 1983 Jul 1;132(1):219-24. doi: 10.1016/0003-2697(83)90451-7.
A new, rapid procedure for purifying bacterial plasmids with high recovery is described. The sequence of operations consists essentially of treatment with alkali, ribonuclease, and proteinase K, followed by chisam extraction and gel filtration on Sephacryl S-1000, and finally a precipitation step using isopropanol at room temperature. The method gives rather good yields of plasmid DNA of high purity, and lends itself to scaling up.
本文描述了一种新的、快速且具有高回收率的细菌质粒纯化方法。操作顺序主要包括用碱、核糖核酸酶和蛋白酶K处理，接着进行奇萨姆萃取和在Sephacryl S - 1000上的凝胶过滤，最后在室温下用异丙醇进行沉淀步骤。该方法能获得相当高产量的高纯度质粒DNA，并且适合扩大规模。
