Hurt E C, Gabellini N, Shahak Y, Lockau W, Hauska G
Arch Biochem Biophys. 1983 Sep;225(2):879-85. doi: 10.1016/0003-9861(83)90101-7.
Isolated cytochrome complexes from different sources like beef heart mitochondria, spinach chloroplasts, cyanobacteria, and photosynthetic bacteria were incorporated into liposomes by sonication as revealed by sucrose density gradient centrifugation and electron microscopy. The reconstituted cytochrome complexes show suppressed rates of quinol-cytochrome c/plastocyanin oxidoreduction which can be stimulated by ionophores and uncouplers. In addition, extra proton translocation out of the vesicles and membrane potential generation during electron transport were observed, suggesting a universal mechanism of electron and proton transport through all the tested cytochrome complexes.
通过蔗糖密度梯度离心和电子显微镜观察发现，从牛肉心线粒体、菠菜叶绿体、蓝细菌和光合细菌等不同来源分离出的细胞色素复合物通过超声处理被整合到脂质体中。重组后的细胞色素复合物显示出喹啉 - 细胞色素c/质体蓝素氧化还原速率受到抑制，而离子载体和解偶联剂可以刺激这种抑制作用。此外，在电子传递过程中还观察到质子从囊泡中额外转运出来以及膜电位的产生，这表明通过所有测试的细胞色素复合物进行电子和质子运输存在一种普遍机制。
