Department of Plant Biology, University of California, 111 Koshland Hall, 94720, Berkeley, CA, USA.
Photosynth Res. 1993 Oct;38(1):73-81. doi: 10.1007/BF00015063.
Cytochrome b 6 f complexes, prepared from spinach and Chlamydomonas thylakoids, have been examined for their content of low molecular weight subunits. The spinach complex contains two prominent low molecular weight subunits of 3.7 and 4.1 kD while a single prominent component of 4.5 kD was present in the Chlamydomonas complex. An estimation of the relative stoichiometry of these subunits suggests several are present at levels approximating one copy per cytochrome complex. The low molecular weight subunits were purified by reversed phase HPLC and N-terminal sequences obtained. Both the spinach and Chlamydomonas cytochrome complexes contain a subunit that is identified as the previously characterized petG gene product (4.8 kD in spinach and 4.1 kD in Chlamydomonas). A second subunit (3.8 kD in spinach and 3.7 kD in Chlamydomonas) appears to be homologous in the two complexes and is likely to be a nuclear gene product. The possible presence of other low molecular weight subunits in these complexes is also considered.
细胞色素 b6f 复合物，由菠菜和衣藻类囊体制备，已经对其低分子量亚基的含量进行了研究。菠菜复合物含有两个明显的低分子量亚基，分子量分别为 3.7 和 4.1kD，而衣藻复合物则存在一个明显的 4.5kD 组成部分。对这些亚基的相对化学计量的估计表明，有几个亚基的含量接近每个细胞色素复合物一个拷贝。通过反相 HPLC 纯化了低分子量亚基，并获得了 N 末端序列。菠菜和衣藻细胞色素复合物都含有一个先前被鉴定为 petG 基因产物（菠菜中为 4.8kD，衣藻中为 4.1kD）的亚基。第二个亚基（菠菜中为 3.8kD，衣藻中为 3.7kD）在两个复合物中似乎是同源的，很可能是核基因产物。还考虑了这些复合物中可能存在其他低分子量亚基的情况。
