Yamada M
Biken J. 1983 Mar;26(1):35-47.
The replication of herpes simplex virus (HSV) in two cell systems derived from rhesus monkeys (LLC-MK2 and DBS-FRhL-2) was studied. In LLC-MK2, the growth of HSV-1 was abortive or extremely limited regardless of the multiplicity of infection, while that of HSV-2 was productive only on infection at high multiplicities. DBS-FRhL-2 cells supported growth of both types of HSV, although growth was highly dependent on the age of monolayers and the infectious dose of virus inocula. Plaques were produced in DBS-FRhL-2 cell monolayers inoculated with HSV-2 but not with HSV-1, although the efficiency of their formation in the former system was much less than in a system of FL and HSV-2. On the other hand, plaques were not produced in LLC-MK2 cell monolayers by either type of HSV. The growth of adapted variants of HSV-1 was also studied. In contrast to the parental strain, these variants replicated well in LLC-MK2 even at a low multiplicity of infection and produced clear plaques in the monolayers. Furthermore, persistent infections of HSV-2 were established in DBS-FRhL-2 cell monolayers under routine culture conditions.
研究了单纯疱疹病毒（HSV）在两种源自恒河猴的细胞系（LLC-MK2和DBS-FRhL-2）中的复制情况。在LLC-MK2中，无论感染复数如何，HSV-1的生长都是流产性的或极其有限的，而HSV-2只有在高感染复数感染时才具有生产性。DBS-FRhL-2细胞支持两种类型HSV的生长，尽管生长高度依赖于单层细胞的年龄和病毒接种物的感染剂量。用HSV-2接种的DBS-FRhL-2细胞单层产生了噬斑，而用HSV-1接种的则未产生，尽管在前者系统中噬斑形成效率远低于在FL和HSV-2系统中。另一方面，两种类型的HSV在LLC-MK2细胞单层中均未产生噬斑。还研究了HSV-1适应变体的生长。与亲本菌株不同，这些变体即使在低感染复数时也能在LLC-MK2中良好复制，并在单层中产生清晰的噬斑。此外，在常规培养条件下，HSV-2在DBS-FRhL-2细胞单层中建立了持续感染。
