Szántó J, Lesso J, Golais F
Acta Virol. 1980 Jun;24(4):244-51.
Herpes simplex virus type 1 (HSV-1) showed limited replication in PK (pig kidney) and XTC-2 (Xenopus laevis frog) cell lines. Virus replication depended on the multiplicity of infection (MOI). At a high MOI, HSV-1 caused a typical cytopathic effect (CPE) in XTC-2 cells but a little marked CPE in PK cells. Pseudorabies virus (PRV) replicated intensively in PK cells (permissive system) but not in XTC-2 cells (nonpermissive system). Both viruses were adsorbed on to PK and XTC-2 cells. In infected PK cells, fluorescent HSV-1 antigen was demonstrated only in the vicinity of the nuclear membrane and in the paranuclear area of the cytoplasm but not in the nuclei. In XTC-2 cells, HSV-1 antigen was demonstrated also in the nuclei. Persistent HSV-1 infection was induced in PK but not in XTC-2 cells; it was of limited duration. PK cells which had lost HSV-1 multiplied further and proved susceptible to infection with HSV-1 or PRV.
1型单纯疱疹病毒（HSV-1）在猪肾（PK）细胞系和非洲爪蟾（Xenopus laevis）蛙XTC-2细胞系中复制受限。病毒复制取决于感染复数（MOI）。在高感染复数时，HSV-1在XTC-2细胞中引起典型的细胞病变效应（CPE），但在PK细胞中CPE不太明显。伪狂犬病病毒（PRV）在PK细胞（允许系统）中大量复制，但在XTC-2细胞（非允许系统）中不复制。两种病毒都能吸附到PK和XTC-2细胞上。在感染的PK细胞中，荧光HSV-1抗原仅在核膜附近和细胞质的核旁区域显示，而不在细胞核中。在XTC-2细胞中，HSV-1抗原也在细胞核中显示。HSV-1在PK细胞中诱导了持续性感染，但在XTC-2细胞中未诱导；这种感染持续时间有限。失去HSV-1的PK细胞进一步增殖，并证明对HSV-1或PRV感染敏感。
