Sánchez de la Peña S, Halberg F, Halberg E, Ungar F, Cornelissen G, Sánchez E, Brown G, Scheving L E, Yunis E G, Vecsei P
Brain Res Bull. 1983 Jul;11(1):117-25. doi: 10.1016/0361-9230(83)90060-6.
In tests of corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1-17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1-17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37 degrees C with 95% O2 and 5% CO2, the media were stored at -20 degrees C until corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1-17 of adrenal corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.
在体外皮质酮生成试验中，松果体水相匀浆（APH）可调节短链促肾上腺皮质激素类似物促肾上腺皮质激素1 - 17（ACTH 1 - 17）对来自不同昼夜节律阶段肾上腺的作用。将处于交错12小时光照/12小时黑暗（LD 12:12）方案下标准化的雌性B6D2F1小鼠的肾上腺和松果体，在每个房间的同一时钟时间获取，以涵盖6个不同的昼夜节律阶段。将每个昼夜节律阶段的肾上腺切成两半，并与来自相同昼夜节律阶段（同相孵育）或其他5个昼夜节律阶段之一（异相孵育）的APH一起孵育。向每个孵育培养基中加入促肾上腺皮质激素1 - 17（0.05 IU）。在37℃、95%氧气和5%二氧化碳条件下孵育4小时后，将培养基储存在-20℃直至进行皮质酮放射免疫分析（RIA）。发现APH对促肾上腺皮质激素1 - 17在体外刺激肾上腺皮质酮生成具有统计学上显著的调节作用。这种APH效应随着昼夜节律阶段有节律地变化，从增强到无作用再到减弱，呈现出所谓的旁侧反馈。
