Haus E, Nicolau G Y, Ghinea E, Dumitriu L, Petrescu E, Sackett-Lundeen L
Health Partners-St. Paul-Ramsey Medical Center, University of Minnesota, USA.
Life Sci. 1996;58(14):PL263-7. doi: 10.1016/0024-3205(96)00079-3.
Adrenals of young adult male mice kept on a LD 12:12 lighting regimen for three weeks prior to study and harvested at four different circadian stages were incubated for 2 hours with 0.4 IU synthetic ACTH in 2 ml Krebs-Ringer buffer (KR), or with 50, 150, and 450 microM of melatonin in KR containing 0.4 IU ACTH. The addition of melatonin to ACTH leads to a dose dependent stimulation of production and/or secretion of DHEA into the incubation medium irrespective of the circadian stage of harvesting of the adrenals. This relationship is of interest in view of the simultaneous decrease of dehydroepiandrosterone and melatonin in the course of aging, and the effects of these compounds upon aging related changes.
在研究前，将处于12小时光照/12小时黑暗（LD 12:12）光照方案下饲养三周的成年雄性小鼠的肾上腺，在四个不同的昼夜节律阶段进行采集，并在2毫升克雷布斯-林格缓冲液（KR）中与0.4国际单位合成促肾上腺皮质激素（ACTH）一起孵育2小时，或者在含有0.4国际单位ACTH的KR中与50、150和450微摩尔褪黑素一起孵育。无论肾上腺采集的昼夜节律阶段如何，向ACTH中添加褪黑素都会导致脱氢表雄酮（DHEA）的产生和/或分泌到孵育培养基中的剂量依赖性刺激。鉴于在衰老过程中脱氢表雄酮和褪黑素同时减少，以及这些化合物对衰老相关变化的影响，这种关系值得关注。
