Butt T R, Sreevalsan T
Exp Cell Res. 1983 Oct 15;148(2):449-59. doi: 10.1016/0014-4827(83)90166-0.
The activity of poly(ADP-ribose) synthetase, a chromatin-bound enzyme, increases when quiescent 3T3 cells are stimulated to proliferate. The elevation of enzymatic activity requires de novo RNA and protein synthesis. Interferon (IFN) or sodium butyrate, when added to quiescent cells at the time of stimulation, suppressed the rise of enzymatic activity as well as initiation of DNA synthesis in cells. However, other DNA synthesis inhibitors like methotrexate, FudR and hydroxyurea had little effect on the elevation of poly(ADP-ribose) synthetase in quiescent cells.
聚（ADP - 核糖）合成酶是一种与染色质结合的酶，当静止的3T3细胞被刺激增殖时，其活性会增加。酶活性的升高需要从头合成RNA和蛋白质。在刺激静止细胞时加入干扰素（IFN）或丁酸钠，会抑制酶活性的升高以及细胞中DNA合成的起始。然而，其他DNA合成抑制剂，如甲氨蝶呤、氟脱氧尿苷和羟基脲，对静止细胞中聚（ADP - 核糖）合成酶的升高影响很小。
